PARTITION OF INSULIN-LIKE GROWTH-FACTOR (IGF)-BINDING SITES BETWEEN THE IGF-I AND IGF-II RECEPTORS AND IGF-BINDING PROTEINS IN THE HUMAN KIDNEY

被引:40
作者
CHIN, E [1 ]
MICHELS, K [1 ]
BONDY, CA [1 ]
机构
[1] NIMH, PHARMACOL SECT, BETHESDA, MD 20892 USA
关键词
D O I
10.1210/jc.78.1.156
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Quantitative ligand binding autoradiography and in situ hybridization were employed to analyze [I-125]insulin-like growth factor-I ([I-125] IGF-I) and [I-125] IGF-II-binding sites in human kidney sections. Binding sites for both ligands were concentrated in the inner medulla and glomeruli, with low levels present in the tubulo-interstitial cortex. Competition with cold IGF-I, IGF-II, and insulin was used to determine nonspecific binding and differentiate binding of ligands to the IGF-I and IGF-II receptors and IGF-binding proteins (IGFBPs). Nonspecific binding was less than 20% of the total for both ligands. Insulin (10(-5) mol/L), which binds to the IGF-I receptor, but not to the IGF-II receptor or IGFBPs, displaced 39 +/- 8% of [I-125]IGF-I binding in glomeruli, 60 +/- 7% in the tubulo-interstitial cortex, and 32 +/- 7% in the medulla. Insulin produced no detectable decrease in [I-125]IGF-II binding in any region. IGF-I (10(-8) mol/L), which binds strongly to IGFBPs, but not appreciably to the IGF-II receptor, produced reductions of 46 +/- 9%, 35 +/- 8%, and 39 +/- 12% in [I-125]IGF-II binding in glomeruli, tubulo-interstitial cortex, and medulla, respectively. In situ hybridization showed that IGFBP-1-5 mRNAs were all expressed in glomeruli. IGFBP-2 mRNA was abundant in medullary collecting duct epithelium, whereas IGFBP-3, -4, and -5 mRNAs were localized in interstitial and vascular cells throughout the kidney. IGF-I and -II receptor mRNAs were widely distributed in renal epithelium. The abundance of local IGFBP gene expression was positively correlated with insulin-nondisplaceable IGF binding in specific kidney regions. In summary, [I-125]IGF-I binding appears to be partitioned largely to IGFBPs in glomeruli and largely to the IGF-I receptor in the tubulointerstitial cortex, with binding in the medulla more evenly divided. The proportion and regional distribution of [I-125]IGF-II binding to IGFBPs are similar, but the balance appears to be primarily associated with the IGF-II, rather than the IGF-I, receptor. Finally, this study shows that [I-125]IGF binding autoradiography combined with in situ hybridization can be used to localize and potentially quantitate expression of IGFBPs in tissue sections.
引用
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页码:156 / 164
页数:9
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