ACID PH CRYSTALLIZATION OF THE BASIC-PROTEIN LYSIN FROM THE SPERMATOZOA OF RED ABALONE (HALIOTIS-RUFESCENS)

被引：4

作者：

DILLER, TC

SHAW, A

STURA, EA

VACQUIER, VD

STOUT, CD

机构：

[1] SCRIPPS RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA

[2] UNIV CALIF SAN DIEGO, SCRIPPS INST OCEANOG, CTR MARINE BIOMED & BIOTECHNOL, LA JOLLA, CA 92093 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1994年 / 50卷

关键词：

D O I：

10.1107/S0907444993013356

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new crystal form of dimeric red lysin, a distinctly basic protein (M(r) = 16070) from the red abalone (Haliotis rufescens), has been obtained using ammonium sulfate as precipitant with a sodium citrate-boric acid-citric acid buffer at pH 4.5. The acid pH crystal form resulted from a study aimed at developing conditions favorable to the sitting-drop vapor-diffusion crystallization of other abalone lysins which do not crystallize at neutral or basic pH conditions. The space group is P222(1) with cell dimensions a = 51.2, b = 47.0, c = 123.8 angstrom and two molecules per asymmetric unit.

引用

页码：620 / 626

页数：7

共 24 条

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