INVIVO DEFINITION OF THE FUNCTIONAL ORIGIN OF REPLICATION (ORI(+)) OF THE PROMISCUOUS PLASMID PLS1

被引：28

作者：

DELSOLAR, G ^{[1
]}

MOSCOSO, M ^{[1
]}

ESPINOSA, M ^{[1
]}

机构：

[1] CSIC, CTR INVEST BIOL, VELAZQUEZ 144, E-28006 MADRID, SPAIN

来源：

MOLECULAR AND GENERAL GENETICS | 1993年 / 237卷 / 1-2期

关键词：

PROMISCUOUS PLASMIDS; SECONDARY STRUCTURES; INITIATION OF REPLICATION; ITERONS;

D O I：

10.1007/BF00282785

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have defined the minimal origin of replication of the plasmid pLS1 leading strand, as comprised within a 247 bp region, by in vivo deletion analyses. Cloning of pLS1 DNA regions containing its oriV(+) into a compatible replicon resulted in weak incompatibility towards pLS1, but only when the cloned fragment included the entire pLS1 oriV(+). Plasmids lacking a functional repB gene (which encodes the pLS1 initiator of replication RepB protein) could be established in Streptococcus pneumoniae only when RepB was supplied in trans. We conclude that all the pLS1-encoded gene products involved in its replication and control are efficient trans-complementing plasmid elements.

引用

页码：65 / 72

页数：8

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