PREPARATIVE AFFINITY-CHROMATOGRAPHY OF PROTEINS

This review describes the role of affinity chromatography in large-scale protein purification. The currently available affinity supports are examined with respect to their use in the preparative scale. Factors that play a major role in successful large-scale affinity purification are discussed. The use of stable synthetic, group-specific ligands, is advocated in the place of fragile, biological counterparts. The use of these affinity media and the validation of affinity chromatography in the process-scale purification of therapeutics are addressed.