DEVELOPMENT AND VALIDATION OF A BIOANALYTICAL METHOD FOR THE QUANTIFICATION OF DILTIAZEM AND DESACETYLDILTIAZEM IN PLASMA BY CAPILLARY ZONE ELECTROPHORESIS

被引：18

作者：

COORS, C ^{[1
]}

SCHULZ, HG ^{[1
]}

STACHE, F ^{[1
]}

机构：

[1] FACHHSCH MUNSTER,ABT STEINFURT,FACHBEREICH CHEM INGN,D-48565 STEINFURT,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 717卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(95)00622-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A CZE method for the quantification of diltiazem and desacetydiltiazem in plasma was developed and validated. Separation was accomplished at pH 2.5 in a 0.044 M phosphate buffer. Sample preparation was performed by liquid-liquid extraction and no interferences with plasma compounds were detected. The calibration graph is linear over the range 5-250 ng/ml with verapamil as internal standard. The precision and accuracy are better than 13% at 5 ng/ml, and better than 10% between 10 and 250 ng/ml. The long-term reliability of the CZE system was checked over a 3-month period. The CZE method is a useful alternative to the already established HPLC method.

引用

页码：235 / 243

页数：9