A new family of lipolytic plant enzymes with members in rice, arabidopsis and maize

被引：99

作者：

Brick, DJ ^{[1
]}

Brumlik, MJ ^{[1
]}

Buckley, JT ^{[1
]}

Cao, JX ^{[1
]}

Davies, PC ^{[1
]}

Misra, S ^{[1
]}

Tranbarger, TJ ^{[1
]}

Upton, C ^{[1
]}

机构：

[1] UNIV VICTORIA, DEPT BIOCHEM & MICROBIOL, VICTORIA, BC V8W 3P6, CANADA

来源：

FEBS LETTERS | 1995年 / 377卷 / 03期

基金：

加拿大自然科学与工程研究理事会;

关键词：

lipase; dbEST; lipolytic; motif; esterase;

D O I：

10.1016/0014-5793(95)01405-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have noted a striking similarity between the sequences of proteins in a novel family of lipases we recently reported [Upton, C. and Buckley, J.T. (1995) Trends Biol, Sci, 20, 178-9] and more than 120 sequences from the database of Expressed Sequence Tags (dbEST) which correspond to at least 30 unique genes from arabidopsis, rice and maize, A cDNA (Arab-1) corresponding to one of these sequences was isolated, sequenced and translated, There was significant similarity to sequences in the new lipase family over the entire open reading frame of Arab-1 and when expressed in E, coli, the gene product was lipolytic, Arab-1 and genes for some of the other plant proteins appear to be differentially expressed, They may play a role in the regulation of lipid metabolism during plant development.

引用

页码：475 / 480

页数：6