MOLECULAR AREAS OF PHOSPHOLIPIDS AS DETERMINED BY H-2 NMR-SPECTROSCOPY - COMPARISON OF PHOSPHATIDYLETHANOLAMINES AND PHOSPHATIDYLCHOLINES

被引：108

作者：

THURMOND, RL ^{[1
]}

DODD, SW ^{[1
]}

BROWN, MF ^{[1
]}

机构：

[1] UNIV ARIZONA,DEPT CHEM,TUCSON,AZ 85721

来源：

BIOPHYSICAL JOURNAL | 1991年 / 59卷 / 01期

关键词：

D O I：

10.1016/S0006-3495(91)82203-2

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The role of lipid diversity in biomembranes is one of the major unsolved problems in biochemistry. One parameter of possible importance is the mean cross-sectional area occupied per lipid molecule, which may be related to formation of nonbilayer structures and membrane protein function. We have used H-2 NMR spectroscopy to compare the properties of 1,2-diperdeuteriopalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE-d62) and 1,2-diperdeuteriopalmitoysl-sn-glycero-3- phosphocholine (DPPC-d62) in the L-alpha phase. We find the DPPE has greater segmental order than DPPC, and that this increased in order is related to the smaller area per acyl chain found for DPPE. Values of the mean cross-sectional chain area are calculated using a simple diamond lattice model for the acyl chain configurational statistics, together with dilatometry data. The results obtained for the mean area per molecule are comparable with those from low angle x-ray diffraction studies.

引用

页码：108 / 113

页数：6