PURIFICATION AND SECONDARY STRUCTURAL-ANALYSIS OF TISSUE INHIBITOR OF METALLOPROTEINASES-1

被引：7

作者：

HODGES, DJ ^{[1
]}

LEE, DC ^{[1
]}

SALTER, CJ ^{[1
]}

REID, DG ^{[1
]}

HARPER, GP ^{[1
]}

CAWSTON, TE ^{[1
]}

机构：

[1] SMITHKLINE BEECHAM PHARMACEUT,WELWYN GARDEN CIT,HERTS,ENGLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1994年 / 1208卷 / 01期

关键词：

TISSUE INHIBITOR METALLOPROTEINASES-1; PROTEIN STRUCTURE; CD; FTIR; NMR; AFFINITY METALLOPROTEINASE PURIFICATION;

D O I：

10.1016/0167-4838(94)90164-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In connective tissue diseases such as rheumatoid arthritis, the matrix metalloproteinases are the primary enzymes involved in tissue degradation. Tissue inhibitor metalloproteinases-1 (TIMP-1) is a specific inhibitor of these enzymes, which is thought to regulate their action in vivo. The structure and function of TIMP-1 may therefore be important as the basis for the rational design of therapeutic agents. This paper describes a simple and effective method for the purification of sufficient quantities of TIMP-1 for spectroscopic studies. Circular dichroism (CD) and Fourier transform infrared (FTIR) spectroscopy have, together, showed TIMP-1 to be mostly in a beta-sheet conformation, with significant amounts of alpha-helix and beta-turn. Two-dimensional nuclear magnetic resonance spectroscopy indicated a correspondingly high proportion of beta-sheet. CD and FTIR have also shown TIMP-1 to have high thermostability.

引用

页码：94 / 100

页数：7

共 36 条

[1] A SYNTHETIC PEPTIDE METALLOPROTEINASE INHIBITOR, BUT NOT TIMP, PREVENTS THE BREAKDOWN OF PROTEOGLYCAN WITHIN ARTICULAR-CARTILAGE INVITRO [J].

ANDREWS, HJ ;

PLUMPTON, TA ;

HARPER, GP ;

CAWSTON, TE .

AGENTS AND ACTIONS, 1992, 37 (1-2) :147-154

[2] THE INTERACTION OF PURIFIED RABBIT BONE COLLAGENASE WITH PURIFIED RABBIT BONE METALLOPROTEINASE INHIBITOR [J].

CAWSTON, TE ;

MURPHY, G ;

MERCER, E ;

GALLOWAY, WA ;

HAZLEMAN, BL ;

REYNOLDS, JJ .

BIOCHEMICAL JOURNAL, 1983, 211 (02) :313-318

[3] PURIFICATION OF RABBIT BONE INHIBITOR OF COLLAGENASE [J].

CAWSTON, TE ;

GALLOWAY, WA ;

MERCER, E ;

MURPHY, G ;

REYNOLDS, JJ .

BIOCHEMICAL JOURNAL, 1981, 195 (01) :159-165

[4] RAPID AND REPRODUCIBLE ASSAY FOR COLLAGENASE USING [ACETYLATED-1-C-14 COLLAGEN [J].

CAWSTON, TE ;

BARRETT, AJ .

ANALYTICAL BIOCHEMISTRY, 1979, 99 (02) :340-345

[5]

CAWSTON TE, 1986, PROTEINASES INFLAMMA, P189

[6] DETERMINATION OF SECONDARY STRUCTURES OF PROTEINS BY CIRCULAR-DICHROISM AND OPTICAL ROTATORY DISPERSION [J].

CHEN, YH ;

YANG, JT ;

MARTINEZ, HM .

BIOCHEMISTRY, 1972, 11 (22) :4120-+

[7] POLYCLONAL AND MONOCLONAL-ANTIBODIES AGAINST HUMAN TISSUE INHIBITOR OF METALLOPROTEINASES (TIMP) AND THE DESIGN OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY TO MEASURE TIMP [J].

CLARK, IM ;

POWELL, LK ;

WRIGHT, JK ;

CAWSTON, TE .

MATRIX, 1991, 11 (02) :76-85

[8] BIOCHEMICAL-CHARACTERIZATION OF MATRILYSIN - ACTIVATION CONFORMS TO THE STEPWISE MECHANISMS PROPOSED FOR OTHER MATRIX METALLOPROTEINASES [J].

CRABBE, T ;

WILLENBROCK, F ;

EATON, D ;

HYNDS, P ;

CARNE, AF ;

MURPHY, G ;

DOCHERTY, AJP .

BIOCHEMISTRY, 1992, 31 (36) :8500-8507

[9] CHARACTERIZATION OF THE FUNCTIONAL DOMAIN OF TISSUE INHIBITOR OF METALLOPROTEINASES-2 (TIMP-2) [J].

DECLERCK, YA ;

YEAN, TD ;

LEE, Y ;

TOMICH, JM ;

LANGLEY, KE .

BIOCHEMICAL JOURNAL, 1993, 289 :65-69

[10]

DECLERCK YA, 1989, J BIOL CHEM, V264, P17445

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