共 21 条
[21]
WILDE CG, 1990, J BIOL CHEM, V265, P2038
USE OF PROTEINASE-3 PURIFIED BY REVERSE-PHASE HPLC TO DETECT AUTOANTIBODIES IN SYSTEMIC VASCULITIS
被引：12
作者：
GASKIN, G
KENDAL, H
COULTHART, A
TURNER, N
PUSEY, CD
机构：
[1] Renal Unit, Department of Medicine, Royal Postgraduate Medical School, London, W12 0NN, Du Cane Road
基金：
英国医学研究理事会;
关键词：
ANTINEUTROPHIL CYTOPLASMIC ANTIBODY;
CHROMATOGRAPHY;
REVERSE-PHASE HIGH PERFORMANCE LIQUID;
PROTEINASE;
3;
ELISA;
VASCULITIS;
D O I：
10.1016/0022-1759(94)00295-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ;
081704 ;
摘要：
The vasculitis autoantigen proteinase 3 was purified from neutrophil primary granules using reverse phase high performance liquid chromatography. It was shown to be free of important contaminants, was enzymatically active and was antigenic to sera containing antineutrophil cytoplasmic antibodies with a cytoplasmic pattern (C-ANCA) by indirect immunofluorescence. Development of an enzyme linked immunosorbent assay showed that this preparation could be used to detect autoantibodies in Wegener's granulomatosis and microscopic polyangiitis. Assays based on reverse phase HPLC-purified proteinase 3 will be valuable in diagnosis and monitoring of treatment, and should increase our understanding of the autoimmune response in primary systemic vasculitis.
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页码：25 / 33
页数：9
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