A NEW METHOD OF STUDYING TEMPERATURE-DEPENDENCE AND THE EFFECT OF MOBILE-PHASE COMPOSITION ON THE RETENTION MECHANISM IN REVERSED-PHASE LIQUID-CHROMATOGRAPHY

被引：20

作者：

GUILLAUME, Y

GUINCHARD, C

机构：

[1] Laboratoire de Chimie Anlytique, UFR des Sciences Médicales et Pharmaceutiques, Place St. Jacques, 25030, Besanˇon Cedex

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1994年 / 17卷 / 13期

关键词：

D O I：

10.1080/10826079408013501

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this work, a rapid procedure to examinate the effect of temperature and eluent composition on thermodynamic properties in high performance liquid chromatography is presented. The use of an experimental design is proposed to study thermodynamic solution property trends for ten Benzodiazepines. Enthalpies and entropies of transfert (mobile to stationary phase) are calculated by evaluation of Van't Hoff plots. For all cases examined enthapies of transfert are negative. These data showed that the entropic contribution to retention becomes more significant as the solvant polarity decreases. Enthalpy-enthropy compensation behavior is tested for varying mobile phase composition.

引用

页码：2809 / 2820

页数：12

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