CYCLIC GMP-MEDIATED INHIBITION OF L-TYPE CA2+ CHANNEL ACTIVITY BY HUMAN NATRIURETIC PEPTIDE IN RABBIT HEART-CELLS

被引:80
作者
TOHSE, N [1 ]
NAKAYA, H [1 ]
TAKEDA, Y [1 ]
KANNO, M [1 ]
机构
[1] HOKKAIDO UNIV,SCH MED,DEPT PHARMACOL,SAPPORO,HOKKAIDO 060,JAPAN
关键词
ATRIAL NATRIURETIC PEPTIDE; CYCLIC GMP; L-TYPE CA2+ CHANNEL; CARDIOMYOCYTE; PATCH CLAMP;
D O I
10.1111/j.1476-5381.1995.tb13316.x
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
1 Effects of atrial natriuretic peptide (ANP) on the L-type Ca2+ channels were examined in rabbit isolated ventricular cells by use of whole-cell and cell-attached configurations of the patch clamp methods. ANP produced a concentration-dependent decrease (10-100 nM) in amplitude of a basal Ca2+ channel current. 2 The inactive ANP (methionine-oxidized ANP, 30 nM) failed to decrease the current. 3 8-Bromo-cyclic GMP (300 mu M), a potent activator of cyclic GMP-dependent protein kinase (PKG), produced the same effects on the basal Ca2+ channel current as those produced by ANP. The cyclic GMP-induced inhibition of the Ca2+ channel current was still evoked in the presence of 1-isobutyl-3-methyl-xanthine, an inhibitor of phosphodiesterase. ANP failed to produce inhibition of the Ca2+ channel current in the presence of 8-bromo-cyclic GMP. 4 In the single channel recording, ANP and 8-bromo-cyclic GMP also inhibited the activities of the L-type Ca2+ channels. Both agents decreased the open probability (NP0) without affecting the unit amplitude. 5 The present results suggest that ANP inhibits the cardiac L-type Ca2+ channel activity through the intracellular production of cyclic GMP and then activation of PKG.
引用
收藏
页码:1076 / 1082
页数:7
相关论文
共 38 条
[11]  
HAN J, 1993, KOREAN J PHYSL, V27, P51
[12]   AGONIST-INDEPENDENT EFFECTS OF MUSCARINIC ANTAGONISTS ON CA2+ AND K+ CURRENTS IN FROG AND RAT CARDIAC-CELLS [J].
HANF, R ;
LI, YX ;
SZABO, G ;
FISCHMEISTER, R .
JOURNAL OF PHYSIOLOGY-LONDON, 1993, 461 :743-765
[13]   OPPOSITE EFFECTS OF CYCLIC-GMP AND CYCLIC-AMP ON CA-2+ CURRENT IN SINGLE HEART-CELLS [J].
HARTZELL, HC ;
FISCHMEISTER, R .
NATURE, 1986, 323 (6085) :273-275
[14]   CALCIUM TOLERANT VENTRICULAR MYOCYTES PREPARED BY PRE-INCUBATION IN A KB MEDIUM [J].
ISENBERG, G ;
KLOCKNER, U .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1982, 395 (01) :6-18
[15]   MOLECULAR-BIOLOGY OF THE NATRIURETIC PEPTIDES AND THEIR RECEPTORS [J].
KOLLER, KJ ;
GOEDDEL, DV .
CIRCULATION, 1992, 86 (04) :1081-1088
[16]   FAILURE OF ATRIOPEPTIN-II TO CAUSE ARTERIAL VASODILATION IN THE CONSCIOUS RAT [J].
LAPPE, RW ;
SMITS, JFM ;
TODT, JA ;
DEBETS, JJM ;
WENDT, RL .
CIRCULATION RESEARCH, 1985, 56 (04) :606-612
[17]   EFFECTS OF ATRIONATRIURETIC FACTOR ON CA2+ CURRENT AND CA(I)-INDEPENDENT TRANSIENT OUTWARD K+ CURRENT IN HUMAN ATRIAL CELLS [J].
LEGRAND, B ;
DEROUBAIX, E ;
COUETIL, JP ;
CORABOEUF, E .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1992, 421 (05) :486-491
[18]  
LEITMAN DC, 1986, J BIOL CHEM, V261, P1650
[19]   CYCLIC-GMP REGULATES THE CA-CHANNEL CURRENT IN GUINEA-PIG VENTRICULAR MYOCYTES [J].
LEVI, RC ;
ALLOATTI, G ;
FISCHMEISTER, R .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1989, 413 (06) :685-687
[20]  
LINCOLN TM, 1983, ADV CYCL NUCL RES<D>, V15, P139