CALCIUM CHANNELS IN EXCITABLE CELLS - DIVERGENT GENOTYPIC AND PHENOTYPIC-EXPRESSION OF ALPHA(1)-SUBUNITS

被引:56
作者
LIEVANO, A [1 ]
BOLDEN, A [1 ]
HORN, R [1 ]
机构
[1] ROCHE INST MOLEC BIOL, NUTLEY, NJ 07110 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1994年 / 267卷 / 02期
关键词
MESSENGER RNA; CALCIUM CURRENT; BARIUM CURRENT; DIHYDROPYRIDINES; OMEGA-CONOTOXIN; DIFFERENTIATION; CELL LINES; FUNNEL WEB SPIDER VENOM;
D O I
10.1152/ajpcell.1994.267.2.C411
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The Ba2+ currents and mRNA levels of four members of the rat brain family of alpha(1)-subunit Ca2+ channel genes were examined and compared in the rat cell lines GH(3) and PC-12 and in the mouse lines NIE-115 and AtT-20. The RNA was measured with ribonuclease protection assays using probes derived from rat brain (rb) Ca2+ channel cDNAs (rbA, rbB, rbC, and rbD), and the Ba2+ currents were studied by whole cell patch-clamp recording. L-, N-, P-, and T-type currents were discriminated by the voltage dependence and pharmacological properties of Ba2+ currents. All cell lines expressed all four rat brain Ca2+ channel genes, except GH(3) cells, which lacked rbB. The functional diversity of Ba2+ currents, however, was quite different among the cell lines. GH(3) cells showed evidence of L- and T-type currents, undifferentiated PC-12 cells of L-type currents, AtT-20 cells of L-, N-, and P-type currents, and undifferentiated NIE-115 cells of a T-type current that was partially blocked by both nifedipine and BAY K 8644. Dimethyl sulfoxide-differentiated NIE-115 cells also had an L-type current. Differentiation of NIE-115 cells caused an increase in the levels of rbB, rbC, and rbD RNAs. Differentiation by nerve growth factor caused an increase in levels of all four genes in PC-12. Our data give further support for the assignment of rbA, rbB, and rbC/rbD gene products as components of P-, N-, and L-type Ca2+ channels, respectively.
引用
收藏
页码:C411 / C424
页数:14
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