P-31-NMR STUDIES OF GLUCOSE AND GLUTAMINE-METABOLISM IN CULTURED-MAMMALIAN-CELLS

被引：15

作者：

SRIPATHMANATHAN, RM ^{[1
]}

BRADDOCK, P ^{[1
]}

BRINDLE, KM ^{[1
]}

机构：

[1] UNIV OXFORD,DEPT BIOCHEM,S PARKS RD,OXFORD OX1 3QU,ENGLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1051卷 / 02期

关键词：

Glucose metabolism; Glutamine metabolism; NMR; !sup]31[!/sup]P; Phosphate-activated glutaminase;

D O I：

10.1016/0167-4889(90)90184-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

31P-NMR measurements of the concentrations of phosphorus-containing metabolites in mammalian cells immobilised and perifused with glucose and glutamine as sole carbon source have shown that the intracellular Pi concentration is significantly higher in cells perifused with glutamine than with glucose. The data are consistent with the proposal that the rate of glutamine utilisation may be controlled by the activity of phosphate-activated glutaminase. © 1990.

引用

页码：131 / 137

页数：7

共 33 条

[21]

MEDINA MA, 1988, BIOCHEM INT, V16, P713

[22] A SIMPLE ANALYSIS OF THE PHOSPHOCREATINE SHUTTLE [J].

MEYER, RA ;

SWEENEY, HL ;

KUSHMERICK, MJ .

AMERICAN JOURNAL OF PHYSIOLOGY, 1984, 246 (05) :C365-C377

[23] APPLICATION OF P-31-NMR SPECTROSCOPY TO THE STUDY OF STRIATED-MUSCLE METABOLISM [J].

MEYER, RA ;

KUSHMERICK, MJ ;

BROWN, TR .

AMERICAN JOURNAL OF PHYSIOLOGY, 1982, 242 (01) :C1-C11

[24] P-31 NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY OF HUMAN RETINOBLASTOMA CELLS - CORRELATIONS WITH METABOLIC INDEXES [J].

MICELI, MV ;

KAN, LS ;

NEWSOME, DA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 970 (03) :262-269

[25]

MOON RB, 1973, J BIOL CHEM, V248, P7276

[26] PHOSPHATE METABOLITES IN LYMPHOID, FRIEND ERYTHROLEUKEMIA, AND HELA-CELLS OBSERVED BY HIGH-RESOLUTION P-31 NUCLEAR MAGNETIC-RESONANCE [J].

NAVON, G ;

NAVON, R ;

SHULMAN, RG ;

YAMANE, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (02) :891-895

[27] P-31-NMR STUDIES OF PHOSPHATE TRANSFER RATES IN T47D HUMAN-BREAST CANCER-CELLS [J].

NEEMAN, M ;

RUSHKIN, E ;

KAYE, AM ;

DEGANI, H .

BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 930 (02) :179-192

[28]

REITZER LJ, 1979, J BIOL CHEM, V254, P2669

[29]

SAUER LA, 1982, CANCER RES, V42, P4090

[30]

TANZER ML, 1959, J BIOL CHEM, V234, P3201

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