PHYSICAL, CHEMICAL AND MICROBIOLOGICAL WATER CHARACTERISTICS ASSOCIATED WITH THE OCCURRENCE OF LEGIONELLA IN COOLING-TOWER SYSTEMS

The occurrence of Legionella in water of 30 cooling tower systems was studied, as well as the relationship of its occurrence with the physical, chemical and microbiological characteristics of the waters. The samples were concentrated by membrane filtration and one part of the concentrate was acid washed. Three types of culture media, MWY, CCVC and BCYEalpha were used. Fourteen (47%) of the cooling systems were Legionella-positive. Numbers of Legionella in the positive systems varied between 50 and 490,000 cfu/l. Legionella pneumophila was the dominant isolate, and the serogroups 6 and 1 were the commonest ones. There was no statistically significant difference in mean water temperature between the Legionella-positive (27-degrees-C) and -negative (24-degrees-C) systems. The total number of bacteria (AODC) was lower in the Legionella-positive than in the negative systems. Also the nutrient concentrations were generally lower in the Legionella-positive cooling systems. The results suggest that different factors regulate the occurrence of Legionella than that of overall bacterial populations in the cooling tower systems.