SELECTIVE PHOTOINHIBITION OF PHOTOSYSTEM-I IN ISOLATED THYLAKOID MEMBRANES FROM CUCUMBER AND SPINACH

被引：84

作者：

SONOIKE, K

机构：

[1] Department of Biological Sciences, Graduate School of Science, University of Tokyo, Bunkyo-ku, Tokyo, 113, Hongo

来源：

PLANT AND CELL PHYSIOLOGY | 1995年 / 36卷 / 05期

关键词：

ACTIVE OXYGEN SPECIES; CHILLING INJURY; PHOTOINHIBITION; PHOTOSYNTHESIS PHOTOSYSTEM I; STRESS EFFECT;

D O I：

10.1093/oxfordjournals.pcp.a078827

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The site of photoinhibition at low temperatures in leaves of a chilling-sensitive plant, cucumber, is photosystem I [Terashima et al. (1994) Planta 193: 300]. As described herein, selective photoinhibition of PSI can also be induced in isolated thylakoid membranes in vitro. Inhibition was observed both at chilling temperatures and at 25 degrees C, and not only in the thylakoid membranes isolated from cucumber, but also in those isolated from a chilling-tolerant plant, spinach. Comparison of these observations in vitro to the earlier results in vivo indicates that (1) photoinhibition of PSI is a universal phenomenon; (2) a mechanism exists to protect PSI in vivo; and (3) the protective mechanism is chilling-sensitive in cucumber. The chilling-sensitive component seems to be lost during the isolation of thylakoid membranes. Very weak light (10-20 mu umol m(-2) s(-1)) was sufficient to cause the inhibition of PSI. About 80% of the oxygen-evolving activity by PSII was maintained even after the activity of PSI had decreased by more than 70%. This is the first report of the selective photoinhibition of PSI in vitro.

引用

页码：825 / 830

页数：6

共 24 条

[21]

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[22]

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[23]

Terashima I., Funayama S., Sonoike K., The site of photoinhibition in leaves of Cucumis sativus L. At low temperatures is photosystem I, not photosystem II, Planta, 193, pp. 300-306, (1994)

[24]

Terashima I., Kashino Y., Katoh S., Exposure of leaves of Cucumis sativus L. To low temperatures in the light causes uncoupling of thylakoids. I. Studies with isolated thyla- koids, Plant Cell Physiol, 32, pp. 1267-1274, (1991)

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