CODING PROPERTIES OF A UNIQUE APURINIC APYRIMIDINIC SITE REPLICATED IN MAMMALIAN-CELLS

被引:51
作者
NETO, JBC [1 ]
CABRAL, REC [1 ]
MARGOT, A [1 ]
LEPAGE, F [1 ]
SARASIN, A [1 ]
GENTIL, A [1 ]
机构
[1] INST RECH SCI CANC,UPR 142,GENET MOLEC LAB,F-94801 VILLEJUIF,FRANCE
关键词
ABASIC SITES; SINGLE-STRANDED VECTOR; MUTAGENESIS; MAMMALIAN CELLS;
D O I
10.1006/jmbi.1994.1457
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The coding properties of abasic sites have been studied in mammalian cells using a single-stranded shuttle vector carrying a unique abasic site. The probe was produced by digestion with the uracil DNA glycosylase of a uracil-containing oligonucleotide which was inserted in the single-stranded vector. After replication in monkey COS7 cells able to support DNA replication of the vector, the plasmid progeny were isolated in bacteria. DNA sequencing of rescued plasmids :showed that replication of abasic sites does not lead to preferential insertion of a given base opposite the non-coding site. The four bases were inserted with a frequency which was not statistically different from a random distribution. It appears therefore that the ‘A rule insertion’ opposite a unique abasic site does not apply, at least with the sequence we used, for an exogeneous single-stranded DNA replicated in mammalian cells. It was not necessary to induce SOS-like conditions by pretreatment of host cells, in order to replicate abasic sites. © 1994 Academic Press Limited.
引用
收藏
页码:416 / 420
页数:5
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