CODING PROPERTIES OF A UNIQUE APURINIC APYRIMIDINIC SITE REPLICATED IN MAMMALIAN-CELLS

被引：51

作者：

NETO, JBC ^{[1
]}

CABRAL, REC ^{[1
]}

MARGOT, A ^{[1
]}

LEPAGE, F ^{[1
]}

SARASIN, A ^{[1
]}

GENTIL, A ^{[1
]}

机构：

[1] INST RECH SCI CANC,UPR 142,GENET MOLEC LAB,F-94801 VILLEJUIF,FRANCE

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1994年 / 240卷 / 05期

关键词：

ABASIC SITES; SINGLE-STRANDED VECTOR; MUTAGENESIS; MAMMALIAN CELLS;

D O I：

10.1006/jmbi.1994.1457

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The coding properties of abasic sites have been studied in mammalian cells using a single-stranded shuttle vector carrying a unique abasic site. The probe was produced by digestion with the uracil DNA glycosylase of a uracil-containing oligonucleotide which was inserted in the single-stranded vector. After replication in monkey COS7 cells able to support DNA replication of the vector, the plasmid progeny were isolated in bacteria. DNA sequencing of rescued plasmids :showed that replication of abasic sites does not lead to preferential insertion of a given base opposite the non-coding site. The four bases were inserted with a frequency which was not statistically different from a random distribution. It appears therefore that the ‘A rule insertion’ opposite a unique abasic site does not apply, at least with the sequence we used, for an exogeneous single-stranded DNA replicated in mammalian cells. It was not necessary to induce SOS-like conditions by pretreatment of host cells, in order to replicate abasic sites. © 1994 Academic Press Limited.

引用

页码：416 / 420

页数：5