ISOLATION OF A PROTEIN THAT IS ESSENTIAL FOR THE FIRST STEP OF NUCLEAR-PROTEIN IMPORT

被引：624

作者：

GORLICH, D

PREHN, S

LASKEY, RA

HARTMANN, E

机构：

[1] UNIV CAMBRIDGE, DEPT ZOOL, CAMBRIDGE CB2 3EJ, ENGLAND

[2] HUMBOLDT UNIV BERLIN, INST BIOCHEM, D-10115 BERLIN, GERMANY

[3] MAX DELBRUCK CTR MOLEC MED, D-13125 BERLIN, GERMANY

来源：

CELL | 1994年 / 79卷 / 05期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0092-8674(94)90067-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have purified a cytosolic protein from Xenopus eggs that is essential for selective protein import into the cell nucleus. The purified protein, named importin, promotes signal-dependent binding of karyophilic proteins to the nuclear envelope. We have cloned, sequenced, and expressed a corresponding cDNA. Importin shows 44% sequence identity with SRP1p, a protein associated with the yeast nuclear pore complex. Complete, signal-dependent import into HeLa nuclei can be reconstituted by combining importin purified from Xenopus eggs or expressed in E. coli with Ran/TC4. Evidence for additional stimulatory factors is provided.

引用

收藏

页码：767 / 778

页数：12

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