FLOW-INJECTION IMMUNOSENSOR FOR THEOPHYLLINE

被引：29

作者：

PALMER, DA

EDMONDS, TE

SEARE, NJ

机构：

[1] Department of Chemistry, Loughborough University of Technology, Loughborough

来源：

ANALYTICAL LETTERS | 1993年 / 26卷 / 07期

关键词：

THEOPHYLLINE; PROTEIN-A; ELECTROCHEMICAL DETECTION; FLOW INJECTION ANALYSIS; ENZYME IMMUNOASSAY;

D O I：

10.1080/00032719308017423

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A heterogeneous on line electrochemical enzyme immunoassay has been developed for the antiasthmatic drug theophylline using a flow injection analysis system containing a protein A immunoreactor to separate antibody bound and unbound theophylline-alkaline phosphatase. In this assay the antibody, label and sample are sequentially immobilised onto the protein A immunoreactor. The antibody-protein A and antibody-protein A label/sample reaction takes place at near neutral pH, and the complexes are eluted at acid pH. p-Aminophenyl phosphate (PAPP) and p-aminophenol (PAP) have been used as the detection system. Experimental variations such as flow rate and reactant concentration have been studied and serum theophylline has been determined by this method and good r.s.d values and % recoveries have been achieved.

引用

页码：1425 / 1439

页数：15

共 13 条

[1]

COOK CE, 1976, RES COMMUN CHEM PATH, V13, P497

[2] RAPID HETEROGENEOUS COMPETITIVE ELECTROCHEMICAL IMMUNOASSAY FOR IGG IN THE PICOMOLE RANGE [J].