COUPLED-COLUMN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF 4 METABOLITES OF POLYCYCLIC AROMATIC-HYDROCARBONS, 1-HYDROXYPHENANTHRENE, 4-HYDROXYPHENANTHRENE AND 9-HYDROXYPHENANTHRENE AND 1-HYDROXYPYRENE, IN URINE

被引：43

作者：

LINTELMANN, J ^{[1
]}

HELLEMANN, C ^{[1
]}

KETTRUP, A ^{[1
]}

机构：

[1] GSF,FORSCHUNGZENTRUM UMWELT & GESUNDHEIT GMBH,INST OKOL CHEM,D-85758 OBERSCHLEISSHEIM,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 660卷 / 01期

关键词：

D O I：

10.1016/0378-4347(94)00267-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A coupled-column high-performance liquid chromatographic method applying system-integrated sample processing on a copper phthalocyanine modified solid-phase material, gradient elution and fluorescence detection for the quantification of 1-, 4- and 9-hydroxyphenanthrene as well as for 1-hydroxypyrene in urine is described. The automated chromatographic system tolerates direct and repeated injection of urine samples. Validation results presented indicate quantitative and matrix-independent recoveries, low imprecisions in within-series and between-series analysis in combination with broad linear working ranges (2-400 nmol/l) and low detection limits (<0.1 nmol/l).

引用

页码：67 / 73

页数：7

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