ISOLATION AND CHARACTERIZATION OF HOMEOBOX-CONTAINING GENES OF CARROT

被引:54
作者
KAWAHARA, R
KOMAMINE, A
FUKUDA, H
机构
[1] JAPAN WOMENS UNIV,DEPT CHEM & BIOL SCI,TOKYO 112,JAPAN
[2] TOHOKU UNIV,FAC SCI,INST BIOL,SENDAI,MIYAGI 980,JAPAN
关键词
CARROT (DAUCUS CAROTA L); 2,4-D; HOMEOBOX; LEUCINE ZIPPER; SOMATIC EMBRYOGENESIS;
D O I
10.1007/BF00019187
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Homeodomains (HDs) are DNA-binding domains that have been well characterized in animals, and HD proteins are thought to be regulators of transcription. To investigate the regulation of gene expression during somatic embryogenesis in carrot, an attempt was made to isolate cDNA clones that encode HD proteins. A cDNA library from carrot somatic embryos was screened with a degenerate oligonucleotide probe that corresponded to a conserved amino acid sequence of HDs, and one cDNA clone (CHB1) encoding an HD protein was isolated. The amino acid sequence deduced from the nucleotide sequence of this clone contained a putative leucine zipper motif adjacent to the anticipated HD. The homeodomain/leucine zipper (HD-Zip) sequence of this cDNA was used for further screening, and five additional independent clones (CHB2 through CHB6) were isolated. Although the I-ID-Zip sequences encoded by these clones were similar to each other, the sequences beyond the HD-Zip regions varied greatly. Transcripts corresponding to CHB1 through CHB6 were expressed at different times during somatic embryogenesis. In particular, transcripts corresponding to CHB2 were expressed in close association with the early development of embryos.
引用
收藏
页码:155 / 164
页数:10
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