A FILTRATION METHOD FOR PLASMID ISOLATION USING MICROTITER FILTER PLATES

被引：5

作者：

RUPPERT, A ^{[1
]}

SZALAY, B ^{[1
]}

VANDENBOOM, D ^{[1
]}

HORST, G ^{[1
]}

KOSTER, H ^{[1
]}

机构：

[1] UNIV HAMBURG,FAC CHEM,DEPT BIOCHEM & MOLEC BIOL,D-20146 HAMBURG,GERMANY

来源：

ANALYTICAL BIOCHEMISTRY | 1995年 / 230卷 / 01期

关键词：

D O I：

10.1006/abio.1995.1447

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the simultaneous isolation of plasmid DNA from as many as 96 Escherichia coli clones in less than 2 h is described. It is based on a modified version of the alkaline lysis procedure originally described by Birnboim and Doly (Nucleic Acids Res. 7, 1513-1523, 1979). The handling of DNA samples is facilitated by the use of microtiter plates with membrane filter bottoms, All centrifugation steps are replaced by filtrations (''the filtration method''). The yield of plasmid DNA from 0.35 ml of an overnight culture is sufficient for restriction analysis of the plasmid clones. Up to 400 nucleotides' readable sequences could be obtained in cycle sequencing reactions with an unmodified sequencing protocol on an automated ABI sequencer. (C) 1995 Academic Press, Inc.

引用

页码：130 / 134

页数：5

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