5-NITROINDOLE AS AN UNIVERSAL BASE ANALOG

被引：143

作者：

LOAKES, D

BROWN, DM

机构：

[1] Medical Research Council, Laboratory of Molecular Biology, Cambridge CB2 2QH, Hills Road

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 20期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1093/nar/22.20.4039

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

4-, 5- and 6-Nitroindole have been investigated and compared with 3-nitropyrrole as universal bases in oligodeoxynucleotides. Of these the 5-nitroindole derivative was found to be superior giving higher duplex stability, and behaving indiscriminately towards each of the four natural bases in duplex melting experiments. 3-Nitropyrrole, whilst not discriminating between the natural bases, was found to lead to considerable destabilisation of the duplexes, particularly when multiple substitutions were made, in contrast to the 5-nitroindole nucleoside.

引用

页码：4039 / 4043

页数：5