PROTEIN-PHOSPHORYLATION IS INDUCED IN TOBACCO CELLS BY THE ELICITOR CRYPTOGEIN

被引:121
作者
VIARD, MP
MARTIN, F
PUGIN, A
RICCI, P
BLEIN, JP
机构
[1] UNIV BOURGOGNE,INRA,PHYTOPHARM PHYTOBIOL CELLULAIRE LAB,F-21034 DIJON,FRANCE
[2] STN BOT & PATHOL VEGETALE,F-06606 ANTIBES,FRANCE
关键词
D O I
10.1104/pp.104.4.1245
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Changes in plasmalemma ion fluxes were observed when tobacco (Nicotiana tabacum) cells were treated with cryptogein, a proteinaceous elicitor from Phytophthora cryptogea. A strong alkalization of the culture medium, accompanied by a leakage of potassium, was induced within a few minutes of treatment. These effects reached a maximum after 30 to 40 min and lasted for several hours. This treatment also resulted in a rapid, but transient, production of activated oxygen species. All these physiological responses were fully sensitive to staurosporine, a known protein kinase inhibitor. Furthermore, a study of protein phosphorylation showed that cryptogein induced a staurosporine-sensitive phosphorylation of several polypeptides. These data suggest that phosphorylated proteins may be essential for the transduction of elicitor signals.
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页码:1245 / 1249
页数:5
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