A SELF-DEFENSE GENE HOMOLOGOUS TO TETRACYCLINE EFFLUXING GENE ESSENTIAL FOR ANTIBIOTIC PRODUCTION IN STREPTOMYCES-AUREOFACIENS

被引：30

作者：

DAIRI, T ^{[1
]}

AISAKA, K ^{[1
]}

KATSUMATA, R ^{[1
]}

HASEGAWA, M ^{[1
]}

机构：

[1] KYOWA HAKKO KOGYO CO LTD, TOKYO RES LABS, MACHIDA, TOKYO 194, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 10期

关键词：

D O I：

10.1271/bbb.59.1835

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By Northern blot analyses with DNA probes carrying 6-demethylchlortetracycline (6-DCT) biosynthetic genes from Streptomyces aureofaciens NRRL3203, a highly expressed gene (tcrC) was detected in a high titer producing mutant derived from the parental strain NRRL3203 by NTG mutagenesis, The analysis of the nucleotide sequence of the 2.8-kb BamHI fragment containing tcrC gene showed that the predicted tcrC gene product is a protein consisting of 512 amino acids. The deduced amino acid sequence had a high level identity with that of the self-defense gene (tet347) of Streptomyces rimosus, known to mediate oxytetracycline efflux. The tcrC gene-inactivated strains generated from strain NRRL3203 by gene replacement had a 90% decrease in the level of resistance to tetracycline and the antibiotic productivity when compared with the parental strain.

引用

页码：1835 / 1841

页数：7

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