MECHANOSENSITIVE ION CHANNELS IN SKELETAL-MUSCLE FROM NORMAL AND DYSTROPHIC MICE

被引:112
作者
FRANCOOBREGON, A [1 ]
LANSMAN, JB [1 ]
机构
[1] UNIV CALIF SAN FRANCISCO,SCH MED,DEPT PHARMACOL,SAN FRANCISCO,CA 94143
来源
JOURNAL OF PHYSIOLOGY-LONDON | 1994年 / 481卷 / 02期
关键词
D O I
10.1113/jphysiol.1994.sp020440
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1. We examined the activity of single mechanosensitive ion channels in recordings from cell-attached patches on myoblasts, differentiated myotubes and acutely isolated skeletal muscle fibres from wild-type and mdx and dy mutant mice. The experiments were concerned with the role of these channels in the pathophysiology of muscular dystrophy. 2. The predominant form of channel activity recorded with physiological saline in the patch electrode arose from an similar to 25 pS mechanosensitive ion channel. Channel activity was similar in undifferentiated myoblasts isolated from all three strains of mice. By contrast, channel activity in mdx myotubes was similar to 3-4 times greater than in either wildtype or dy myotubes and arose from a novel mode of mechanosensitive gating. 3. Single mechanosensitive channels in acutely isolated flexor digitorum brevis fibres had properties indistinguishable from those of muscle cells grown in tissue culture. The channel open probability in mdx fibres was similar to 2 times greater than the activity recorded from wild-type fibres. The overall level of activity in fibres, however, was roughly an order of magnitude smaller than in myoblasts or myotubes. 4. Histological examination of the flexor digitorum brevis fibres from mds mice showed no evidence of myonecrosis or regenerating fibres, suggesting that the elevated channel activity in dystrophin-deficient muscle precedes the onset of fibre degeneration. 5. An early step in the dystrophic process of the mdx mouse, which leads to pathophysiological Ca2+ entry, may be an alteration in the mechanisms that regulate mechanosensitive ion channel activity.
引用
收藏
页码:299 / 309
页数:11
相关论文
共 36 条