CHARACTERIZATION OF 6 NEW LOCI WITHIN THE SWINE MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-III REGION

被引：16

作者：

NUNES, M

PEELMAN, L

VAIMAN, M

BOURGEAUX, N

CHARDON, P

机构：

[1] INRA,CEA,DSV,DPTE,RADIOBIOL APPL LAB,F-78352 JOUY EN JOSAS,FRANCE

[2] STATE UNIV GHENT,B-9820 MERELBEKE,BELGIUM

来源：

MAMMALIAN GENOME | 1994年 / 5卷 / 10期

关键词：

D O I：

10.1007/BF00411456

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A search for new potential coding sequences was conducted within two overlapping cosmid genomic DNA clusters of about 170 and 45 kb from the swine major histocompatibility complex class III region. The sequences were detected with various probes, including pools of swine cDNA, homologous and heterologous genomic sequences, and synthetic oligonucleotides. The 170 kb cluster was centered on the tumor necrosis factor genes (TNF), and the 45 kb cluster contained the heat-shock protein 70 genes (HSP70). The TNF cluster revealed the presence of five new genes: lymphotoxin beta, BAT1, BAT2, BAT3, and a sequence related to DNA-binding factors. No sequence homologous to B144 was found in the TNF cluster, although other unidentified coding sequences may be present in this cluster. The HSP70 cluster contained a gene identified as BAT6, that is, tRNA-valyl synthetase. These results provide new evidence that the genomic maps of these various genes in the TNF and HSP70 sub-regions are similar in swine and human.

引用

页码：616 / 622

页数：7

共 26 条

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