SELECTIVE EFFECTS OF TPA AND IL-1 ON PROTEIN-PHOSPHORYLATION IN MURINE THYMOCYTES

被引：13

作者：

AVISSAR, S

STENZEL, KH

NOVOGRODSKY, A

机构：

[1] BEILINSON MED CTR, ROGOFF WELLCOME MED RES INST, IL-49100 PETACH TIKVA, ISRAEL

[2] TEL AVIV UNIV, SACKLER SCH MED, TEL AVIV, ISRAEL

来源：

CELLULAR IMMUNOLOGY | 1985年 / 96卷 / 02期

关键词：

D O I：

10.1016/0008-8749(85)90378-8

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Protein kinase C activity was demonstrated in murine thymocytes and the effects of TPA and IL-1 on this enzyme were studied. TPA, but not IL-1, could substitute for diacylglycerol in protein kinase C activation. Although TPA and IL-1 are both potent comitogens for murine thymocytes they markedly differ in their effects on protein phosphorylation and protein kinase C activation. Treatment of intact thymocytes with TPA resulted in a marked increase in the phosphorylation of an endogenous protein with Mr .apprx. 44,000. Enhanced phosphorylation of this protein was also observed when protein kinase C was activated in thymocytes extracts. In contrast to TPA. IL-1 neither induced phosphorylation of the 44,000-Da protein nor activated protein kinase C. The data suggests that protein kinase C does not mediate the comitogenic effect of IL-1 in murine thymocytes.

引用

页码：462 / 471

页数：10

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