Agrobacterium tumefaciens-mediated transformation of broccoli (Brassica oleracea var italica) and cabbage (B-oleracea var capitata)

被引：91

作者：

Metz, TD ^{[1
]}

Dixit, R ^{[1
]}

Earle, ED ^{[1
]}

机构：

[1] CORNELL UNIV,DEPT PLANT BREEDING,ITHACA,NY 14853

来源：

PLANT CELL REPORTS | 1995年 / 15卷 / 3-4期

关键词：

D O I：

10.1007/BF00193738

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Transgenic broccoli (Brassica oleracea var. italica) was produced by two Agrobacterium tumefaciens-mediated transformation methods. One used flowering stalk explants from mature plants; the other used hypocotyl and petiole explants from in vitro-grown seedlings. Several hundred transformants containing a Bacillus thuringiensis a-endotoxin gene (CryIA(c)-type) and the neomycin phosphotransferase gene were recovered, Rooted transformants were obtained in as little as 3 months using seedling explants. Transgenic cabbage was also obtained by the seedling explant method. Parameters important for high efficiency regeneration and transformation rates included use of a tobacco nurse cell layer, sealing of petri dishes with a porous surgical tape instead of Parafilm, preculture of seedling explants and appropriate length of co-cultivation with Agrobacterium. Advantages and disadvantages of each transformation procedure are discussed.

引用

页码：287 / 292

页数：6

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