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LOCALIZATION OF RABPHILIN-3A ON THE SYNAPTIC VESICLE

被引：100
作者：
MIZOGUCHI, A
YANO, Y
HAMAGUCHI, H
YANAGIDA, H
IDE, C
ZAHRAOUI, A
SHIRATAKI, H
SASAKI, T
TAKAI, Y
机构：
[1] FAC MED LARIBOISIERE,INSERM,U248,F-75010 PARIS,FRANCE
[2] NATL INST PHYSIOL SCI,DEPT CELL PHYSIOL,OKAZAKI,AICHI 444,JAPAN
[3] OSAKA UNIV,SCH MED,DEPT BIOCHEM & MOLEC BIOL,SUITA,OSAKA 565,JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 202卷 / 03期
关键词：
D O I：
10.1006/bbrc.1994.2063
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Rabphilin-3A is a putative target protein for Rab3A small GTP-binding protein which is implicated in neurotransmitter release. Rabphilin-3A is expressed mainly in brain, but its subcellular localization remains to be clarified. Immunohistochemical analysis has revealed that Rabphilin-3A is most abundant in the synaptic area of the rat cerebellum, retina, and neuromuscular junction. Ultrastructural analysis of the neuromuscular junction using the immunogold method indicates that Rabphilin-3A is localized on the synaptic vesicle. Subcellular fractionation analysis of rat brain has shown that Rabphilin-3A is most highly concentrated in the purified synaptic vesicle fraction. These results indicate that Rabphilin-3A is localized on the synaptic vesicle in the presynapse. (C) 1994 Academic Press, Inc.
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收藏
页码：1235 / 1243
页数：9
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