GLUTAMIC-ACID DECARBOXYLASE OF EMBRYONIC AVIAN RETINA CELLS IN CULTURE - REGULATION BY GAMMA-AMINOBUTYRIC-ACID (GABA)

被引:16
作者
DEMELLO, FG
HOKOC, JN
VENTURA, ALM
GARDINO, PF
机构
[1] Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Rio de Janeiro, 21944, bloco G
关键词
GAMMA-AMINOBUTYRIC ACID (GABA); GLUTAMIC ACID DECARBOXYLASE (GAD) IMMUNOCYTOCHEMISTRY; RETINA CELLS;
D O I
10.1007/BF00734811
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
1. Retina-cell aggregate cultures expressed glutamate decarboxylase activity (L-glutamate 1-carboxylase; EC 4.1.1.15) as a function of culture differentiation. 2. Glutamic acid decarboxylase (GAD) activity was low in the initial phases of culture and increased eight-fold until culture day 7, remaining high up to day 13 (last stage studied). 3. The addition of GABA to the culture medium 24 h after cell seeding almost totally prevented the expression of GAD activity. 4. In association with decreased enzyme activity, aggregates exposed to GABA did not display immunoreactivity for GAD, suggesting that GAD molecules were either lost from GABAergic neurons or significantly altered with GABA treatment. 5. Control, untreated aggregates showed intense GAD immunoreactivity in neurons. Positive cell bodies were characterized by a thin rim of labeled cytoplasm with thickest labeling at the emergence of the main neurite. 6. Heavily labeled patches were also observed throughout the aggregates, possibly reflecting regions enriched in neurites. 7. The GABA-mediated reduction of GAD immunoreactivity was a reversible phenomenon and could be prevented by picrotoxin.
引用
收藏
页码:485 / 496
页数:12
相关论文
共 22 条
[1]   HISTIOTYPIC ORGANIZATION AND CELL-DIFFERENTIATION IN RAT RETINAL REAGGREGATE CULTURES [J].
AKAGAWA, K ;
HICKS, D ;
BARNSTABLE, CJ .
BRAIN RESEARCH, 1987, 437 (02) :298-308
[2]   IDENTIFICATION AND CHARACTERIZATION OF CELL-TYPES ACCUMULATING GABA IN RAT RETINAL CULTURES USING CELL TYPE SPECIFIC MONOCLONAL-ANTIBODIES [J].
AKAGAWA, K ;
BARNSTABLE, CJ .
BRAIN RESEARCH, 1987, 408 (1-2) :154-162
[3]   PATTERN OF EXPRESSION OF GLUTAMIC-ACID DECARBOXYLASE MESSENGER-RNA IN THE DEVELOPING RAT-BRAIN [J].
BOND, RW ;
JANSEN, KR ;
GOTTLIEB, DI .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (09) :3231-3234
[4]   RETINAL GABA NEURONS - LOCALIZATION IN VERTEBRATE SPECIES USING AN ANTISERUM TO RABBIT BRAIN GLUTAMATE-DECARBOXYLASE [J].
BRANDON, C .
BRAIN RESEARCH, 1985, 344 (02) :286-295
[5]  
De Mello F G, 1976, J Neurochem, V27, P847
[6]   GABA-MEDIATED CONTROL OF GLUTAMATE-DECARBOXYLASE (GAD) IN CELL AGGREGATE CULTURE OF CHICK-EMBRYO RETINA [J].
DEMELLO, FG .
DEVELOPMENTAL BRAIN RESEARCH, 1984, 14 (01) :7-13
[7]   L-GLUTAMATE EVOKED RELEASE OF GABA FROM CULTURED AVIAN RETINA CELLS DOES NOT REQUIRE GLUTAMATE RECEPTOR ACTIVATION [J].
DEMELLO, MCF ;
KLEIN, WL ;
DEMELLO, FG .
BRAIN RESEARCH, 1988, 443 (1-2) :166-172
[8]  
DONASCIMENTO JLM, 1985, J NEUROCHEM, V45, P1820
[9]   DEVELOPMENTAL IMMUNOREACTIVITY FOR GABA AND GAD IN THE AVIAN RETINA - POSSIBLE ALTERNATIVE PATHWAY FOR GABA SYNTHESIS [J].
HOKOC, JN ;
VENTURA, ALM ;
GARDINO, PF ;
DEMELLO, FG .
BRAIN RESEARCH, 1990, 532 (1-2) :197-202
[10]  
LOWRY OH, 1951, J BIOL CHEM, V193, P265