ETHOXYRESORUFIN-O-DEETHYLASE (EROD) ACTIVITY IN FLATFISH - FAST DETERMINATION WITH A FLUORESCENCE PLATE-READER

被引：93

作者：

EGGENS, ML ^{[1
]}

GALGANI, F ^{[1
]}

机构：

[1] IFREMER,DERO,DEPT MILIEU & RESSOURCES,F-44037 NANTES 01,FRANCE

来源：

MARINE ENVIRONMENTAL RESEARCH | 1992年 / 33卷 / 03期

关键词：

D O I：

10.1016/0141-1136(92)90149-G

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Ethoxyresorufin-O-deethylase (EROD) is one of the model reactions of the cytochrome P-450IA1 mediated monooxygenase (MO) system. It is often used to detect the effects of increased concentrations of planar aromatic microcontaminants. The comparison of a new and faster method for measuring the EROD-activity using a plate-reader with the conventional method showed an almost equal sensitivity and precision between the two methods. Characteristic in the use of the plate-reader method is that the protein concentration of the (diluted) sample has to be in the range of 0.9-3 mg ml-1. In this range the EROD-kinetics are linear for at least 8 min. The compactness and rigidity of the equipment and the speed of analysis makes the plate-reader method very suitable for research on board of a research vessel.

引用

页码：213 / 221

页数：9

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