THE REV2 GENE OF SACCHAROMYCES-CEREVISIAE - CLONING AND DNA-SEQUENCE

被引：12

作者：

AHNE, F ^{[1
]}

BAUR, M ^{[1
]}

ECKARDTSCHUPP, F ^{[1
]}

机构：

[1] GSF FORSCH ZENTRUM UMWELT & GESUNDHEIT,INST STRAHLENBIOL,INGOLSTADTER LANDSTR 1,W-8042 NEUHERBERG,GERMANY

来源：

CURRENT GENETICS | 1992年 / 22卷 / 04期

关键词：

YEAST; DNA-REPAIR; MUTATION-DEFICIENT MUTANT; NUCLEOTIDE-BINDING CONSENSUS;

D O I：

10.1007/BF00317921

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The REV2 gene of Saccharomyces cerevisiae was cloned and sequenced; it contains an open reading frame of 1986 bp with a coding potential of 662 amino acids. Interruption of the chromosomal REV2 gene by integrating the URA3 gene coupled with partial deletion of the 3' terminal region produced viable haploid rev2-DELTA mutants. This indicates that the REV2 gene is non-essential for growth. The rev2-DELTA mutant is slightly more UV-sensitive than strains carrying various rev2 alleles (rev2-1, rev2x, rad5-1, rad5-8). The putative Rev2 protein is probably a globular protein containing a highly conserved nucleotide-binding site and two zinc-finger domains.

引用

页码：277 / 282

页数：6

共 56 条

[1] MOLECULAR ANALYSIS OF THE REV2 GENE OF SACCHAROMYCES-CEREVISIAE - A REVIEW [J].