THE ENDOGENOUS BACILLUS-SUBTILIS (NATTO) PLASMIDS PTA1015 AND PTA1040 CONTAIN SIGNAL PEPTIDASE-ENCODING GENES - IDENTIFICATION OF A NEW STRUCTURAL MODULE ON CRYPTIC PLASMIDS

被引：63

作者：

MEIJER, WJJ ^{[1
]}

DEJONG, A ^{[1
]}

BEA, G ^{[1
]}

WISMAN, A ^{[1
]}

TJALSMA, H ^{[1
]}

VENEMA, G ^{[1
]}

BRON, S ^{[1
]}

MAARTEN, J ^{[1
]}

VANDIJL, JM ^{[1
]}

机构：

[1] GRONINGEN BIOMOLEC SCI & BIOTECHNOL INST,DEPT GENET,9751 NN HAREN,NETHERLANDS

来源：

MOLECULAR MICROBIOLOGY | 1995年 / 17卷 / 04期

关键词：

D O I：

10.1111/j.1365-2958.1995.mmi_17040621.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Various strains of Bacillus subtilis (natto) contain small cryptic plasmids that replicate via the rolling-circle mechanism. Like plasmids from other Gram-positive bacteria, these plasmids are composed of several distinct structural modules. A new structural module was identified on the B. subtilis plasmids pTA1015 and pTA1040. It is composed of two genes: one specifies an unidentified protein with a putative signal peptide; and the other (sipP) specifies a functional type I signal peptidase (SPase). The homologous, but non-identical, sipP genes of the two plasmids are the first identified plasmid-specific SPase-encoding genes, With respect to structure and activity, the corresponding enzymes (denoted SipP) are highly similar to the chromosomally encoded SPase, SipS, of B. subtilis and several newly identified SPases of other bacilli. Our findings suggest that plasmid-encoded SPases have evolved because, under certain conditions, SPase can be a limiting factor for protein secretion in B. subtilis.

引用

页码：621 / 631

页数：11

共 46 条

[1] ON THE CATALYTIC MECHANISM OF PROKARYOTIC LEADER PEPTIDASE-1 [J].

BLACK, MT ;

MUNN, JGR ;

ALLSOP, AE .

BIOCHEMICAL JOURNAL, 1992, 282 :539-543

[2] EVIDENCE THAT THE CATALYTIC ACTIVITY OF PROKARYOTE LEADER PEPTIDASE DEPENDS UPON THE OPERATION OF A SERINE-LYSINE CATALYTIC DYAD [J].

BLACK, MT .

JOURNAL OF BACTERIOLOGY, 1993, 175 (16) :4957-4961

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4] ULTRAVIOLET INACTIVATION AND EXCISION-REPAIR IN BACILLUS-SUBTILIS .1. CONSTRUCTION AND CHARACTERIZATION OF A TRANSFORMABLE EIGHTFOLD AUXOTROPHIC STRAIN AND 2 ULTRAVIOLET-SENSITIVE DERIVATIVES [J].

BRON, S ;

VENEMA, G .

MUTATION RESEARCH, 1972, 15 (01) :1-+

[5] STABILITY FUNCTION IN THE BACILLUS-SUBTILIS PLASMID PTA1060 [J].

BRON, S ;

BOSMA, P ;

VANBELKUM, M ;

LUXEN, E .

PLASMID, 1987, 18 (01) :8-15

[6] PLASMID INSTABILITY AND MOLECULAR-CLONING IN BACILLUS-SUBTILIS [J].

BRON, S ;

MEIJER, W ;

HOLSAPPEL, S ;

HAIMA, P .

RESEARCH IN MICROBIOLOGY, 1991, 142 (7-8) :875-883

[7]

BRON S, 1990, MOL BIOL METHODS BAC, P75

[8] THE PMTL NIC-CLONING VECTORS .1. IMPROVED PUC POLYLINKER REGIONS TO FACILITATE THE USE OF SONICATED DNA FOR NUCLEOTIDE SEQUENCING [J].

CHAMBERS, SP ;

PRIOR, SE ;

BARSTOW, DA ;

MINTON, NP .

GENE, 1988, 68 (01) :139-149

[9] STRUCTURAL AND GENETIC ANALYSES OF A PAR LOCUS THAT REGULATES PLASMID PARTITION IN BACILLUS-SUBTILIS [J].

CHANG, S ;

CHANG, SY ;

GRAY, O .

JOURNAL OF BACTERIOLOGY, 1987, 169 (09) :3952-3962

[10] HIGH-FREQUENCY TRANSFORMATION OF BACILLUS-SUBTILIS PROTOPLASTS BY PLASMID DNA [J].

CHANG, S ;

COHEN, SN .

MOLECULAR & GENERAL GENETICS, 1979, 168 (01) :111-115

← 1 2 3 4 5 →