DETERMINATION OF THE MYCOTOXIN CYCLOPIAZONIC ACID BY ENZYME-IMMUNOASSAY

被引：24

作者：

HAHNAU, S ^{[1
]}

WEILER, EW ^{[1
]}

机构：

[1] RUHR UNIV BOCHUM,LEHRSTUHL PFLANZENPHYSIOL,UNIV STR 150,W-4630 BOCHUM,GERMANY

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1991年 / 39卷 / 10期

关键词：

D O I：

10.1021/jf00010a041

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Antisera highly selective for the mycotoxin cyclopiazonic acid (CPA) were raised in rabbits. A competitive enzyme-linked immunosorbent assay (ELISA) for CPA, with use of alkaline phosphatase as tracer enzyme, was developed. The assay measures from 30 pg to 2 ng of CPA and showed an interassay, between-day variability of 14 % and an intraassay variability of 3.7 %. An efficient selection technique was devised that allows detection of CPA, even at low levels, in fungal strains growing as agar surface cultures. No precleaning of the samples is required. The technique allows efficient monitoring for fungal isolates producing CPA.

引用

页码：1887 / 1891

页数：5