REGULATION OF CHOLECYSTOKININ SECRETION BY CALCIUM-DEPENDENT CALMODULIN KINASE-II - DIFFERENTIAL-EFFECTS OF PHENYLALANINE AND CAMP

被引:16
作者
PRPIC, V
BASAVAPPA, S
LIDDLE, RA
MANGEL, AW
机构
[1] DUKE UNIV,MED CTR,DEPT MED,DIV GASTROENTEROL,DURHAM,NC 27710
[2] DURHAM VET AFFAIRS MED CTR,DURHAM,NC 27710
关键词
D O I
10.1006/bbrc.1994.1871
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The release of cholecystokinin was investigated in STC-1 cells, an intestinal cholecystokinin-secreting cell line. Fifteen minute incubation of cells with the amino acid, L-phenylalanine (20 mM), or the phosphodiesterase inhibitor, IBMX (100 mu M), stimulated cholecystokinin secretion. stimulation of secretion by both agents was associated with an increase in cytosolic calcium and was inhibited by the calcium channel blocker, diltiazem (10 mu M). The calcium-calmodulin kinase II inhibitor, KN-62 (1.4 mu M), markedly reduced IBMX-stimulated secretion, but had no effect on phenylalanine-mediated activity. KN-62 also inhibited IBMX-induced increases in cytosolic calcium, suggesting that cAMP may activate diltiazem-sensitive calcium channels by a calmodulin-dependent process. (C) 1994 Academic Press, Inc.
引用
收藏
页码:1483 / 1489
页数:7
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