Electroporation-induced damage in mammalian cell DNA

被引：60

作者：

Meaking, WS ^{[1
]}

Edgerton, J ^{[1
]}

Wharton, CW ^{[1
]}

Meldrum, RA ^{[1
]}

机构：

[1] UNIV BIRMINGHAM,SCH BIOCHEM,BIRMINGHAM B15 2T,W MIDLANDS,ENGLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1995年 / 1264卷 / 03期

关键词：

electroporation; DNA break; mammalian cell; electric charge; current duration;

D O I：

10.1016/0167-4781(95)00177-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Electroporation induced damage in the DNA of HL60 cells has been investigated by alkaline elution techniques. DNA damage is minimised by reducing the total charge applied (i.e., voltage X capacitance). Reduction of either of these electrical parameters, however, compromises the induced permeability of the cells to small molecules. The data presented concerning the effects of voltage and capacitance on DNA damage and the permeability of cells can be used to specify optimum conditions for electroporation in which DNA damage is minimised. The duration for which the current is applied can be seen to have a significant effect on the level of DNA damage. A modest temperature rise may occur when an electric charge is passed through electroporation buffer, but this event alone does not induce DNA damage in cells. The effect of voltage upon the permeability of HL60 cells to fluorescent-labelled molecules of varying molecular weight is reported.

引用

页码：357 / 362

页数：6

共 29 条

[11]

KINSOTA K, 1979, BIOCHIM BIOPHYS ACTA, V554, P479

[12]

KUBINIEC RT, 1990, BIOTECHNIQUES, V155, P92

[13] P-31 NMR ANALYSIS OF MEMBRANE PHOSPHOLIPID ORGANIZATION IN VIABLE, REVERSIBLY ELECTROPERMEABILIZED CHINESE-HAMSTER OVARY CELLS [J].

LOPEZ, A ;

ROLS, MP ;

TEISSIE, J .

BIOCHEMISTRY, 1988, 27 (04) :1222-1228

[14] STIMULATION OF ENDOGENOUS ENDONUCLEASE ACTIVITY IN HEPATOCYTES EXPOSED TO OXIDATIVE STRESS [J].

MCCONKEY, DJ ;

HARTZELL, P ;

NICOTERA, P ;

WYLLIE, AH ;

ORRENIUS, S .

TOXICOLOGY LETTERS, 1988, 42 (02) :123-130

[15] THE KINETICS AND MECHANISM OF REPAIR OF UV INDUCED DNA-DAMAGE IN MAMMALIAN-CELLS - THE USE OF CAGED NUCLEOTIDES AND ELECTROPORATION TO STUDY SHORT-TIME COURSE EVENTS IN DNA-REPAIR [J].

MELDRUM, RA ;

MEAKING, WS ;

WHARTON, CW .

NUCLEIC ACIDS RESEARCH, 1994, 22 (07) :1234-1241

[16] ELECTROCHEMOTHERAPY POTENTIATION OF ANTITUMOR EFFECT OF BLEOMYCIN BY LOCAL ELECTRIC PULSES [J].

MIR, LM ;

ORLOWSKI, S ;

BELEHRADEK, J ;

PAOLETTI, C .

EUROPEAN JOURNAL OF CANCER, 1991, 27 (01) :68-72

[17] INTRODUCTION OF DEFINITE AMOUNTS OF NONPERMEANT MOLECULES INTO LIVING CELLS AFTER ELECTROPERMEABILIZATION - DIRECT ACCESS TO THE CYTOSOL [J].

MIR, LM ;

BANOUN, H ;

PAOLETTI, C .

EXPERIMENTAL CELL RESEARCH, 1988, 175 (01) :15-25

[18] INDUCTION AND REPAIR OF DNA DOUBLE-STRAND BREAKS [J].

NEVALDINE, B ;

LONGO, JA ;

KING, GA ;

VILENCHIK, M ;

SAGERMAN, RH ;

HAHN, PJ .

RADIATION RESEARCH, 1993, 133 (03) :370-374

[19]

PEREZ CF, 1984, RADIAT RES, V104, P200

[20] THE RELATIONSHIP BETWEEN RADIATION-INDUCED DNA DOUBLE-STRAND BREAKS AND CELL KILL IN HAMSTER V79 FIBROBLASTS IRRADIATED WITH 250 KVP X-RAYS, 2.3 EV NEUTRONS OR PU-238 ALPHA-PARTICLES [J].

PRISE, KM ;

DAVIES, S ;

MICHAEL, BD .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1987, 52 (06) :893-902

← 1 2 3 →