GIBBERELLIC-ACID STIMULATES LIPID-METABOLISM IN BARLEY ALEURONE PROTOPLASTS

被引:10
作者
ARNALTE, ME
CORNEJO, MJ
BUSH, DS
JONES, RL
机构
[1] UNIV VALENCIA,FAC FARM,DEPT PLANT BIOL,E-46010 VALENCIA,SPAIN
[2] UNIV CALIF BERKELEY,DEPT PLANT BIOL,BERKELEY,CA 94720
基金
美国国家科学基金会;
关键词
ALEURONE PROTOPLASTS; BARLEY (HORDEUM-VULGARE L); LIPID BODIES (OIL BODIES); LIPID DYES; LIPID MOBILIZATION; PHOSPHOLIPIDS;
D O I
10.1016/0168-9452(91)90091-L
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Changes in the lipid composition of barley aleurone protoplasts following incubation in the presence or absence of gibberellic acid (GA) were studied and correlated with cell development. Analysis of neutral lipids in protoplast extracts and purified lipid bodies provide evidence for the strong effect of GA on the mobilization of storage lipids. In vivo protoplast staining with the membrane marker N-phenyl-1-naphthylamine (NPN) and phospholipid determinations of protoplast extracts show an increase in membrane lipids during protoplast incubation. GA stimulates phospholipid metabolism by increasing the synthesis of phosphatidyl ethanolamine and phosphatidyl choline, the major phospholipid constituents of the endoplasmic reticulum.
引用
收藏
页码:223 / 232
页数:10
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