SEQUENCE-SPECIFIC [H-1]NMR RESONANCE ASSIGNMENTS AND SECONDARY STRUCTURE IDENTIFICATION FOR 1-ZINC AND 2-ZINC FINGER CONSTRUCTS FROM SWI5 - A HYDROPHOBIC CORE INVOLVING 4 INVARIANT RESIDUES

Complete [1H]NMR resonance assignments are presented for the second of the three zinc fingers from SWI5 as it appears in both 1- and 2-finger constructs. Signals from finger 2 are unaffected by the presence or absence of finger 1, showing that the protein has a modular construction. The structure of finger 2 comprises a helix running from N56 to Q67, and a region approximating to an anti-parallel β-sheet running from Y42 to F53. These features combine to produce a hydrophobic core in the structure involving the invariant residues Y42, F53, L59 and H62. © 1990.