SEQUENCE-SPECIFIC [H-1]NMR RESONANCE ASSIGNMENTS AND SECONDARY STRUCTURE IDENTIFICATION FOR 1-ZINC AND 2-ZINC FINGER CONSTRUCTS FROM SWI5 - A HYDROPHOBIC CORE INVOLVING 4 INVARIANT RESIDUES

被引：50

作者：

NEUHAUS, D

NAKASEKO, Y

NAGAI, K

KLUG, A

机构：

[1] MRC Laboratory for Molecular Biology, Cambridge, CB2 2QH England, Hills Road

来源：

FEBS LETTERS | 1990年 / 262卷 / 02期

关键词：

DNA binding; Secondary structure; Sequence-specific resonance assignment; Zinc finger; [!sup]1[!/sup]H]Nuclear magnetic resonance; 2-dimensional;

D O I：

10.1016/0014-5793(90)80184-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Complete [1H]NMR resonance assignments are presented for the second of the three zinc fingers from SWI5 as it appears in both 1- and 2-finger constructs. Signals from finger 2 are unaffected by the presence or absence of finger 1, showing that the protein has a modular construction. The structure of finger 2 comprises a helix running from N56 to Q67, and a region approximating to an anti-parallel β-sheet running from Y42 to F53. These features combine to produce a hydrophobic core in the structure involving the invariant residues Y42, F53, L59 and H62. © 1990.

引用

页码：179 / 184

页数：6

共 21 条

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