MULTIPLE POSITIVE AND NEGATIVE CIS-ACTING ELEMENTS OF THE STA2 GENE REGULATE GLUCOAMYLASE SYNTHESIS IN SACCHAROMYCES-CEREVISIAE

被引：18

作者：

LAMBRECHTS, MG

PRETORIUS, IS

DAGUANNO, VS

SOLLITTI, P

MARMUR, J

机构：

[1] UNIV STELLENBOSCH,DEPT MICROBIOL,STELLENBOSCH 7600,SOUTH AFRICA

[2] UNIV STELLENBOSCH,INST BIOTECHNOL,STELLENBOSCH 7600,SOUTH AFRICA

[3] YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT BIOCHEM,BRONX,NY 10461

来源：

GENE | 1994年 / 146卷 / 02期

关键词：

RECOMBINANT DNA; STA10; REPRESSION; UPSTREAM ACTIVATING/REPRESSING SEQUENCES; YEAST;

D O I：

10.1016/0378-1119(94)90286-0

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Expression of the glucoamylase-encoding gene (STA2) in Saccharomyces cerevisiae was previously shown to be regulated transcriptionally by both positive and negative factors. The objective of this work was to identify the cis-acting elements responsible for STA2 transcriptional activation as well as the transcriptional repressor effects of STA10 and MATa/MAT alpha. We identified two upstream activation regions (UAS). Three repressor regions responsive to STA10-mediated repression were identified, as well as two regions for down-regulation of STA2 expression. MATa/MAT alpha repression appears to effect STA2 expression either downstream from the translational start site or, indirectly, since no functional a1/alpha 2responsive sequence was identified in the promoter region.

引用

页码：137 / 144

页数：8

共 35 条

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