(TG)N UNCOVERS A SEX-SPECIFIC HYBRIDIZATION PATTERN IN CATTLE

被引:27
作者
KASHI, Y
IRAQI, F
TIKOCHINSKI, Y
RUZITSKY, B
NAVE, A
BECKMANN, JS
FRIEDMANN, A
SOLLER, M
GRUENBAUM, Y
机构
[1] HEBREW UNIV JERUSALEM, INST LIFE SCI, DEPT GENET, IL-91904 JERUSALEM, ISRAEL
[2] AGR RES ORG, VOLCANI CTR, DEPT PLANT GENET & BREEDING, IL-50250 BET DAGAN, ISRAEL
关键词
D O I
10.1016/0888-7543(90)90515-V
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Screening of a bovine genomic library with the human minisatellite 33.6 probe uncovered a family of clones that, when used to probe Southern blots of bovine genomic DNA digested with the restriction enzyme HaeIII or MboI, revealed sexually dimorphic, but otherwise virtually monomorphic, patterns among the larger DNA fragments to which they hybridized. Characterization of one of these clones revealed that it contains different minisatellite sequences. The sexual dimorphism hybridization pattern observed with this clone was found to be due to multiple copies of two tandemly interspersed repeats: the simple sequence (TG)n and a previously undescribed 29-bp sequence. Both repeats appear to share many genomic loci including autosomal loci. In contrast, Southern analysis of AluI- or HinfI-digested bovine DNA with the (TG)n repeat used as a probe yielded substantial polymorphism. These results show that (i) different minisatellites can be found in a cluster, (ii) both simple and more complex repeated sequences other than the simple quaternary (GATA)n repeat can be sexually dimorphic, and (iii) simple repeats can reveal substantial polymorphism. © 1990.
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页码:31 / 36
页数:6
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