THE PYRUVATE-DEHYDROGENASE COMPLEX OF SACCHAROMYCES-CEREVISIAE IS REGULATED BY PHOSPHORYLATION

被引：17

作者：

JAMES, AG ^{[1
]}

COOK, RM ^{[1
]}

WEST, SM ^{[1
]}

LINDSAY, JG ^{[1
]}

机构：

[1] UNIV GLASGOW, INST BIOMED & LIFE SCI, DIV BIOCHEM & MOLEC BIOL, GLASGOW G12 8QQ, LANARK, SCOTLAND

来源：

FEBS LETTERS | 1995年 / 373卷 / 02期

关键词：

YEAST; PYRUVATE DEHYDROGENASE COMPLEX; PHOSPHORYLATION; INACTIVATION AND REACTIVATION;

D O I：

10.1016/0014-5793(95)01020-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mitochondria mere isolated from Saccharomyces cerevisiae grown on different carbon sources prior to incubation with [gamma-P-32]ATP, A major 46,000-M(r) phosphoprotein, corresponding in M(r) value to the El alpha subunit of the yeast pyruvate dehydrogenase complex (PDC), was detected only in mitochondria isolated from cells grown on a fermentable carbon source such as galactose, Immunoprecipitation with subunit-specific antiserum to the E1 component of mammalian or yeast PDC confirmed the identity of this polypeptide. PDC activity in isolated yeast mitochondria could be inactivated in an ATP-dependent fashion and reactivated in the presence of Ca2+ ions.

引用

页码：111 / 114

页数：4

共 18 条

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