AUTOANTIBODIES FROM PATIENTS WITH PRIMARY BILIARY-CIRRHOSIS PREFERENTIALLY REACT WITH THE AMINO-TERMINAL DOMAIN OF NUCLEAR-PORE COMPLEX GLYCOPROTEIN GP210

被引:49
作者
WESIERSKAGADEK, J [1 ]
HOHENAUER, H [1 ]
HITCHMAN, E [1 ]
PENNER, E [1 ]
机构
[1] UNIV VIENNA,DEPT INTERNAL MED 4,A-1090 VIENNA,AUSTRIA
关键词
D O I
10.1084/jem.182.4.1159
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Patients with primary biliary cirrhosis frequently develop autoantibodies directed to gp210, a major glycoprotein of the nuclear pore complex. This protein contains a large glycosylated cisternal domain, a single transmembrane segment, and a short cytoplasmic tail. It has been previously shown that autoantibodies from primary biliary cirrhosis patients exclusively react with the cytoplasmic tail. We demonstrate that autoantibodies against gp210 recognize at least two different epitopes. 4 out of 12 anti-gp210 positive sera reacted with the fragment consisting of the cytoplasmic tail, and 8 sera targeted a novel epitope located within the large glycosylated lumenal domain. Moreover, our data prove that carbohydrate moieties are an essential part of this novel epitope. We propose, therefore, that future screening assays should be performed with antigens possessing both epitopes to detect all sera with anti-gp210 specificity.
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收藏
页码:1159 / 1162
页数:4
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