DIFFERENTIAL POLARIZATION IMAGING .5. NUMERICAL APERTURE EFFECTS AND THE CONTRIBUTION OF PREFERENTIAL SCATTERING AND ABSORPTION TO THE CIRCULAR-DICHROISM IMAGES

被引：9

作者：

FINZI, L ^{[1
]}

ULIBARRI, L ^{[1
]}

BUSTAMANTE, C ^{[1
]}

机构：

[1] UNIV NEW MEXICO,DEPT CHEM,ALBUQUERQUE,NM 87131

来源：

BIOPHYSICAL JOURNAL | 1991年 / 59卷 / 06期

关键词：

D O I：

10.1016/S0006-3495(91)82334-7

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Chiral objects which scatter and absorb preferentially left versus right circularly polarized light give rise to bright-field circular dichroism (CD) images containing contributions from both these two phenomena. These contributions are separated and characterized mathematically, and the effect of the dimensions of the chiral object on their relative magnitude is discussed. CD images of the long-range chiral organization of the thylakoid membranes in chloroplasts are obtained at two different wavelengths to illustrate the diverse wavelength dependence of the preferential absorption and scattering contributions to the images. The bright field CD images not only depend on the magnitude and sign of the preferential scattering and preferential absorption contributions, but also on the numerical aperture of the lens used. This dependence is obtained formally and a method to extract the angle dependent preferential scattering contributions to the images is presented. The validity of this method is confirmed

引用

页码：1183 / 1193

页数：11

共 15 条

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