DNA-SEQUENCES REQUIRED FOR TRANSLATIONAL FRAMESHIFTING IN PRODUCTION OF THE TRANSPOSASE ENCODED BY IS1

被引：18

作者：

SEKINE, Y ^{[1
]}

OHTSUBO, E ^{[1
]}

机构：

[1] UNIV TOKYO, INST APPL MICROBIOL, YAYOI 1-1-1, BUNKYO KU, TOKYO 113, JAPAN

来源：

MOLECULAR AND GENERAL GENETICS | 1992年 / 235卷 / 2-3期

关键词：

ADENINE RUN; COINTEGRATION; SECONDARY STRUCTURE OF MESSENGER RNA; TERMINATION CODON; TRANSFER RNALYS;

D O I：

10.1007/BF00279377

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The transposase encoded by insertion sequence IS1 is produced from two out-of-phase reading frames (insA and B'-insB) by translational frameshifting, which occurs within a run of six adenines in the - 1 direction. To determine the sequence essential for frameshifting, substitution mutations were introduced within the region containing the run of adenines and were examined for their effects on frameshifting. Substitutions at each of three (2nd, 3rd and 4th) adenine residues in the run, which are recognized by tRNA(Lys) reading insA, caused serious defects in frameshifting, showing that the three adenine residues are essential for frameshifting. The effects of substitution mutations introduced in the region flanking the run of adenines and in the secondary structures located downstream were, however, small, indicating that such a region and structures are not essential for frameshifting. Deletion of a region containing the termination codon of insA caused a decrease in beta-galactosidase activity specified by the lacZ fusion plasmid in frame with B'-insB. Exchange of the wild-type termination codon of insA for a different one or introduction of an additional termination codon in the region upstream of the native termination codon caused an increase in beta-galactosidase activity, indicating that the termination codon in insA affects the efficiency of frameshifting.

引用

页码：325 / 332

页数：8

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