CHARACTERIZATION OF BINDING AND TNF-ALPHA-INDUCING ABILITY OF CHITOSANS ON MONOCYTES - THE INVOLVEMENT OF CD14

被引：84

作者：

OTTERLEI, M ^{[1
]}

VARUM, KM ^{[1
]}

RYAN, L ^{[1
]}

ESPEVIK, T ^{[1
]}

机构：

[1] UNIV TRONDHEIM,INST BIOTECHNOL,N-7034 TRONDHEIM,NORWAY

来源：

VACCINE | 1994年 / 12卷 / 09期

关键词：

CHITOSAN; TNF-ALPHA PRODUCTION; MONOCYTES;

D O I：

10.1016/0264-410X(94)90292-5

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Chitosans with different chemical composition were found to induce TNF-a production from human monocytes. Their ability to induce TNF-alpha was found to be highly dependent on neutral-solubility and molecular weight. Monoclonal antibodies against CD14 inhibited TNF-alpha production from monocytes stimulated with neutral-soluble chitosans. Binding studies indicated that lipopolysaccharides (LPS) and neutral-soluble chitosans share a binding site on monocytes which involves CD14. TNF-alpha production from monocytes stimulated with chitosans was dependent on serum. LPS-binding protein (LBP) enhanced the chitosan-induced TNF-alpha production only to a minor degree, suggesting that serum proteins other than LBP play an important role in the stimulatory effect.

引用

页码：825 / 832

页数：8

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