COMPARATIVE-ANALYSIS OF HUMAN AND CHICKEN TRANSFORMING GROWTH-FACTOR-BETA-2 AND FACTOR-BETA-3 PROMOTERS

The promoters for chicken transforming growth factor-beta-2 (TGF-beta-2) and TGF-beta-3 were cloned and sequenced to study the regulation of these genes. The promoters are GC-rich and lie within CpG islands. Several putative DNA regulatory sequence motifs were identified in the 5'-flanking regions, including matches to particular recognition sequences for several nuclear factors found in other genes. A comparison of chicken and human TGF-beta-2 promoters revealed a 111 bp conserved sequence surrounding the major transcription start site. Two regions of sequence homology were detected in the 5'-flanking regions of chicken and human TGF-beta-3 genes: an 86 bp sequence surrounding the major transcription start and a 156 bp sequence in the 5'-untranslated region. No DNA sequence homology was detected between TGF-beta-1, -beta-2 or -beta-3 promoters. The conserved region near the major transcription start sites in both the TGF-beta-2 and TGF-beta-3 genes, however, does show some structural homology; both promoters contain short conserved sequences that resemble TATA box, cyclic AMP-responsive element and AP-2 sequence motifs, cis-acting elements we believe may be important for promoter activity.