Cloning by Polymerase Chain Reaction of a New Mouse TGF-beta, mTGF-beta 3

被引：53

作者：

Denhez, Fabienne ^{[1
]}

Lafyatis, Robert ^{[1
]}

Kondaiah, Paturu ^{[1
]}

Roberts, Anita B. ^{[1
]}

Sporn, Michael B. ^{[1
]}

机构：

[1] NIH, Lab Chemoprevent, Bldg 41,Room C629, Bethesda, MD 20892 USA

来源：

GROWTH FACTORS | 1990年 / 3卷 / 02期

关键词：

TGF-beta; PCR; development;

D O I：

10.3109/08977199009108276

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Two TGF-beta s, TGF-beta I and 2, have previously been isolated from the mouse. Here we report the isolation of a murine TGF-beta 3 cDNA. RNAs extracted from 15-day-old mouse embryos and several mouse cell lines were reverse-transcribed. These cDNA mixtures were used as substrates for polymerase chain-reaction amplifications, using oligonucleotides designed on the basis of known human and chicken TGF-beta 3 sequences, including the initiation and stop codons. Several overlapping cDNAs containing either the amino-terminal domain or the carboxy-terminal domain, as well as the complete 1.2-kb coding region of the mouse TGF-beta 3 cDNA were obtained. The mouse TGF-beta 3 coding region is 1230 nucleotides long and codes for a 410 amino acid polypeptide very similar to its human counterpart. This cDNA hybridizes to a unique 3.5-kb RNA and is differentially expressed in various mouse tissues and at different embryonic stages.

引用

页码：139 / 146

页数：8

共 21 条

[1] TRANSFER OF PROTEINS ACROSS MEMBRANES .1. PRESENCE OF PROTEOLYTICALLY PROCESSED AND UNPROCESSED NASCENT IMMUNOGLOBULIN LIGHT-CHAINS ON MEMBRANE-BOUND RIBOSOMES OF MURINE MYELOMA
BLOBEL, G
DOBBERSTEIN, B
[J]. JOURNAL OF CELL BIOLOGY, 1975, 67 (03) : 835 - 851
[2] GENOMIC SEQUENCING
CHURCH, GM
GILBERT, W
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07): : 1991 - 1995
[3] DAVIS LG, 1986, BASIC METHODS MOL BI, P143
[4] DERYNCK R, 1986, J BIOL CHEM, V261, P4377
[5] A NEW TYPE OF TRANSFORMING GROWTH FACTOR-BETA, TGF-BETA-3
DERYNCK, R
LINDQUIST, PB
LEE, A
WEN, D
TAMM, J
GRAYCAR, JL
RHEE, L
MASON, AJ
MILLER, DA
COFFEY, RJ
MOSES, HL
CHEN, EY
[J]. EMBO JOURNAL, 1988, 7 (12) : 3737 - 3743
[6] ROLE OF TRANSFORMING GROWTH FACTOR-BETA IN THE DEVELOPMENT OF THE MOUSE EMBRYO
HEINE, UI
MUNOZ, EF
FLANDERS, KC
ELLINGSWORTH, LR
LAM, HYP
THOMPSON, NL
ROBERTS, AB
SPORN, MB
[J]. JOURNAL OF CELL BIOLOGY, 1987, 105 (06) : 2861 - 2876
[7] COMPLEMENTARY DEOXYRIBONUCLEIC-ACID CLONING OF A NOVEL TRANSFORMING GROWTH FACTOR-BETA MESSENGER RIBONUCLEIC-ACID FROM CHICK-EMBRYO CHONDROCYTES
JAKOWLEW, SB
DILLARD, PJ
KONDAIAH, P
SPORN, MB
ROBERTS, AB
[J]. MOLECULAR ENDOCRINOLOGY, 1988, 2 (08) : 747 - 755
[8] KONDAIAH P, 1990, J BIOL CHEM, V265, P1089
[9] LEHNERT SA, 1988, DEVELOPMENT, V104, P263
[10] VGR-1, A MAMMALIAN GENE RELATED TO XENOPUS VG-1, IS A MEMBER OF THE TRANSFORMING GROWTH FACTOR-BETA GENE SUPERFAMILY
LYONS, K
GRAYCAR, JL
LEE, A
HASHMI, S
LINDQUIST, PB
CHEN, EY
HOGAN, BLM
DERYNCK, R
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (12) : 4554 - 4558

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