Cloning by Polymerase Chain Reaction of a New Mouse TGF-beta, mTGF-beta 3

被引：53

作者：

Denhez, Fabienne ^{[1
]}

Lafyatis, Robert ^{[1
]}

Kondaiah, Paturu ^{[1
]}

Roberts, Anita B. ^{[1
]}

Sporn, Michael B. ^{[1
]}

机构：

[1] NIH, Lab Chemoprevent, Bldg 41,Room C629, Bethesda, MD 20892 USA

来源：

GROWTH FACTORS | 1990年 / 3卷 / 02期

关键词：

TGF-beta; PCR; development;

D O I：

10.3109/08977199009108276

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Two TGF-beta s, TGF-beta I and 2, have previously been isolated from the mouse. Here we report the isolation of a murine TGF-beta 3 cDNA. RNAs extracted from 15-day-old mouse embryos and several mouse cell lines were reverse-transcribed. These cDNA mixtures were used as substrates for polymerase chain-reaction amplifications, using oligonucleotides designed on the basis of known human and chicken TGF-beta 3 sequences, including the initiation and stop codons. Several overlapping cDNAs containing either the amino-terminal domain or the carboxy-terminal domain, as well as the complete 1.2-kb coding region of the mouse TGF-beta 3 cDNA were obtained. The mouse TGF-beta 3 coding region is 1230 nucleotides long and codes for a 410 amino acid polypeptide very similar to its human counterpart. This cDNA hybridizes to a unique 3.5-kb RNA and is differentially expressed in various mouse tissues and at different embryonic stages.

引用

页码：139 / 146

页数：8

共 21 条

[11] PATTERNS OF EXPRESSION OF MURINE VGR-1 AND BMP-2A RNA SUGGEST THAT TRANSFORMING GROWTH FACTOR-BETA-LIKE GENES COORDINATELY REGULATE ASPECTS OF EMBRYONIC-DEVELOPMENT
LYONS, KM
PELTON, RW
HOGAN, BLM
[J]. GENES & DEVELOPMENT, 1989, 3 (11) : 1657 - 1668
[12] THE TGF-BETA FAMILY OF GROWTH AND DIFFERENTIATION FACTORS
MASSAGUE, J
[J]. CELL, 1987, 49 (04) : 437 - 438
[13] MURINE TRANSFORMING GROWTH FACTOR-BETA-2 CDNA SEQUENCE AND EXPRESSION IN ADULT TISSUES AND EMBRYOS
MILLER, DA
LEE, A
PELTON, RW
CHEN, EY
MOSES, HL
DERYNCK, R
[J]. MOLECULAR ENDOCRINOLOGY, 1989, 3 (07) : 1108 - 1114
[14] A TRANSCRIPT FROM A DROSOPHILA PATTERN GENE PREDICTS A PROTEIN HOMOLOGOUS TO THE TRANSFORMING GROWTH-FACTOR-BETA FAMILY
PADGETT, RW
STJOHNSTON, RD
GELBART, WM
[J]. NATURE, 1987, 325 (6099) : 81 - 84
[15] ROBERTS AB, 1988, RECENT PROG HORM RES, V44, P157
[16] ARG-GLY-ASP - A VERSATILE CELL RECOGNITION SIGNAL
RUOSLAHTI, E
PIERSCHBACHER, MD
[J]. CELL, 1986, 44 (04) : 517 - 518
[17] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS
SANGER, F
NICKLEN, S
COULSON, AR
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) : 5463 - 5467
[18] Spom M.B., 1987, J CELL BIOL, V105, P1039
[19] TENDIJKE P, 1988, P NATL ACAD SCI USA, V85, P4715
[20] Transforming Growth Factor Beta-1 in Acute Myocardial Infarction in Rats
Thompson, Nancy L.
Bazoberry, Fernando
Speir, Edith H.
Casscells, Ward
Ferrans, Victor J.
Flanders, Kathleen C.
Kondaiah, Patljru
Geiser, Andrew G.
Sporn, Michael B.
[J]. GROWTH FACTORS, 1988, 1 (01) : 91 - 99

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